How does formalin fixation affect nucleic acid integrity?
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How does formalin fixation affect nucleic acid integrity?
Formalin fixation preserves tissue, but it comes at a cost to nucleic acids. Mary Friedli Malone explains how formalin creates covalent cross-links within and between nucleic acids and proteins, leading to fragmentation, chemical modification, and degraded DNA and RNA that can complicate downstream applications like PCR and sequencing.
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